I. ENVIRONMENTAL BIOTECHNOLOGY
Anaerobic
digestion and co-digestion of poultry waste: challenges,
process limits, and emerging opportunities
Ivan Simeonov, Venelin Hubenov
5-29
II. SPACE TECHNOLOGY
AND ENVIRONMENTAL MONITORING
Rosen Iliev,
Boyko Ranguelov
30-37
III. RENEWABLE AND ALTERNATIVE
ENERGY SOURCES AND BIOFUELS
Valorization of taro
(Colocasia
esculenta L.) agricultural waste into energy
dense briquettes: a sustainable fuel
quality assessment
Arum
Widyasmara, Erna Styani, Chairil Anwar, Winny Iftari, Kurnia
Widhi Astuti, Poppy Sri Lestari
38-44
IV. ECOLOGICAL AND SUSTAINABLE AGRICULTURE
Nasir Uddin Soykot, Md. Sharieutullah, Deen Mohammad Deepo,
A. K. M. Ruhul Amin
45-57
V. RADIATION ECOLOGY
Modeling
and analysis of flux and power distribution of HTR-10
reactor core
Mohamed A. Alzamly, Hesham
Elkatib 58-67
VI. MAN AND BIOSPHERE
Research
on the Alpha Omega Water® Crystal Instrument: results and analyses with FT–IR,
EIS, NMR, and DFT
Ignat Ignatov, Yordan Marinov
68-74
Tsvetan Iliev 75-82
VII. MICROORGANISMS
AND ENVIRONMENT
In vitro antimicrobial
activity of L-malic acid-based composition
Teodora
Popova, Mila Kaleva, Fabio Huether, Alexander
I. Ignatov, Ignat Ignatov
83-92
VIII.
NOTABLE SCIENTISTS
Life
and scientific contributions of geneticist Doncho Kostov
Alexander Alexandrov,
Borislav Yangyozov
93-94
IX. SCIENTIFIC FORUMS
95
IXth
International Conference Ecological Engineering and
Environment Protection (EEEP'2027) with Youth Scientific
Session,
19 - 22 May 2027, Varna, Bulgaria
doi.org/10.32006/eeep.2026.1.0529
ANAEROBIC DIGESTION
AND CO-DIGESTION OF POULTRY WASTE: CHALLENGES, PROCESS
LIMITS, AND EMERGING OPPORTUNITIES
Ivan Simeonov1, Venelin Hubenov1,2
1
The
Stephan Angeloff Institute of Microbiology, Bulgarian
Academy of Sciences, Sofia,
Bulgaria
2 Competence Center “Clean
Technologies for a Sustainable Environment – Waters,
Wastes, Energy
for Circular Economy”, Sofia, Bulgaria
1 Contact
details of corresponding author
e-mail:
issim@abv.bg; issim@microbio.bas.bg
Abstract. The
poultry industries are growing rapidly due to its
availability of lean meat with low cholesterol content.
However, the problems also coming along with the poultry
manure production as a waste from poultry industries. Òhe
increase in the demand of poultry products have led to fast
and concentrated growth of the poultry industry, which
deliver superabundant manure supplies. Poultry waste is one
of the best organic manure and also much valuable resource.
At present the poultry farming supply vital sprig of animal
protein and also the most freely reared livestock species
amongst the all livestock species. In spite of its huge
benefits like production of meat, eggs and job generations
it frequently creates some environmental risks that
adversely affect both humans and animals, because the
poultry farming is the main cause of offensive odor, water,
soil, and air pollution. The conversion of poultry waste
into biogas and biofertilizer is considered one of the most
effective waste management and recycling strategies.
The
aim of this manuscript is to deliver a comprehensive
overview of the state of the art, the technical feasibility
and some perspectives of the anaerobic digestion and
anaerobic co-digestion of poultry waste for biogas
production including our own results in this field.
Keywords: poultry waste, environmental pollution,
chicken manure, AD, AcoD, complex mixtures, biogas
production, biofertilizer
Rosen Iliev,
Boyko Ranguelov
Space
Research and Technology Institute, Bulgarian Academy of
Sciences, Sofia, Bulgaria
Abstract: This study investigates the surface complexity of the
Protonilus Mensae region in the northern hemisphere of Mars
using an integrated approach based on fractal dimension (FD)
and lacunarity analysis. Digital elevation model (DEM) data
derived from the Mars Orbiter Laser Altimeter (MOLA), with a
spatial resolution of 463 m, were analyzed using Python-based
algorithms. Surface roughness was analyzed using a
variogram-based fractal method. The results show that the
topography of Protonilus Mensae exhibits fractal behavior,
with FD values ranging from 2.11 to 2.70 and a mean value of
2.35. Spatial patterns of FD reveal predominantly low to
moderate surface complexity. Lacunarity analysis was
employed to characterize spatial heterogeneity and the
distribution of topographic gaps. (Lacunarity means empty
spaces (gaps) in DEM with similar fractal dimensions but
different surface view.) The results show a strong
dependence of lacunarity on elevation, with maximum
heterogeneity occurring at intermediate elevations
associated with fretted (not smooth) terrain. Lower and
higher elevations exhibit reduced lacunarity. The combined
fractal and lacunarity results suggest that the present-day
morphology of Protonilus Mensae represents a degraded
landscape shaped by a balance between roughness-enhancing
processes and smoothing processes. This
integrated quantitative approach provides new insights into
the geomorphological evolution of not flat terrains on Mars.
Keywords: Protonilus Mensae, Mars, fractal dimension,
lacunarity, variogram, smooth terrains.
1Department of Chemical Analysis,
Polytechnic of AKA Bogor, Bogor 16154, Indonesia.
2 Department of Industrial Waste
Treatment, Polytechnic of AKA Bogor, Bogor 16154,
Indonesia.
3Department of Food Industry Quality Assurance, Polytechnic of AKA Bogor, Bogor
16154, Indonesia.
Abstract. This study investigates the potential of taro
(Colocasia esculenta L.) peel, a significant
agricultural waste, as a raw material for high-quality
solid fuel briquettes. To optimize characteristics,
briquettes were pre-treated by oven-drying (90ºC-120ºC,
30-60 min) and assessed via proximate analysis, DSC, and
Ignition Rate testing. Results confirmed successful
property upgrades, identifying 120ºC for 45 minutes as
optimal for energy concentration. This condition yielded
the highest Fixed Carbon (55.08%) and total thermal output
(1,689.176 J/g by DSC), plus the maximum Ignition Rate
(0.0008778 g/second), confirming superior combustion
kinetics. The 120ºC variant's superior energy content and
confirmed thermal stability (onset 206.840ºC) establish it
as the most promising fuel. Findings validate the
technical feasibility of transforming taro peel waste into
an energy-dense solid fuel, demonstrating a viable
strategy for sustainable waste management and renewable
energy generation.
Keywords: Taro Peel Waste,
Solid Fuel Briquettes, Valorization, Fixed Carbon, Thermal
Stability, Ignition Rate, DSC
Nasir Uddin Soykot1,*, Md.
Sharieutullah1, Deen Mohammad Deepo2
& Dr.
A. K. M. Ruhul Amin3
1 Institute
of Seed Technology, Sher-E-Bangla Agricultural University,
Dhaka, Bangladesh
2 Department of Agricultural
Science, Daffodil International University, Dhaka,
Bangladesh
3 Dept.
of Agronomy, Sher-E-Bangla Agricultural University, Dhaka,
Bangladesh
Correspondence:
nasiruddinsoykot.sau.74@gmail.com*
Abstract. A field experiment was conducted during the Aman
season (July–December 2019) in a subtropical region of
Bangladesh to address the prevailing challenge of low
national T. Aman rice yield and the common farmer practice
of using over-aged seedlings. The study aimed to determine
the optimal seedling age and genotype combination for
maximizing both grain yield and seed quality. Three rice
varieties (BRRI dhan32, BRRI dhan62, and BRRI dhan80) were
evaluated across four transplanting ages (20, 25, 30, and 35
days) using a two-factor Randomized Complete Block Design
(RCBD). Results demonstrated that the variety BRRI dhan32
was statistically superior, yielding the highest grain (4.68
t/ha) and exhibiting the best seed germination (88.33%),
primarily due to its robust yield components, including a
greater number of effective tillers and filled seeds per
panicle. Seedlings transplanted at 30 days old (A3)
significantly outperformed other ages, promoting optimal
growth and development and resulting in a maximum yield of
4.83 t/ha and germination of 92.01%. Crucially, the
interaction between BRRI dhan32 and 30-day-old seedlings (V1A3)
produced the highest combined performance, recording an
exceptional grain yield of 5.12 t/ha, superior germination
(92.86%), and the highest vigor index (2152.49), largely
attributable to enhanced 1000-seed weight. This research
conclusively recommends cultivating BRRI dhan32 with
30-day-old seedlings as a practical and effective strategy
to significantly boost the productivity and quality of T.
Aman rice, thereby enhancing national food security in
subtropical environments.
Keywords: Seeding, Yield Quality, T. Aman,
Harvesting, Rice.
Mohamed A. Alzamly1, Hesham
Elkatib2
1Fuel Cycle Safety Department,
Nuclear and Radiological Safety Research Center,
Egyptian Atomic Energy Authority, Cairo, Egypt
2Reactor Department, Nuclear Research
Center, Egyptian Atomic Energy Authority, Cairo, Egypt
Abstract. MCNP6 computer code
is used to model HTR-10 core reactor. UO2
fuel is used. We predict the Flux and power distribution
for normal core loaded by UO2 fuel pebbles of
the reference HTR-10 reactor. The MCNP model consisted
of the reactor structure which included the graphite
reflector, the borated carbon bricks surrounding it and
the pebble-bed core. The results show an analogue
between the thermal neutron flux distribution and the
power distribution, where the thermal neutrons are
responsible for causing the fission, and hence power
generation. The thermal neutron flux and power
generation have its maximum value at the core center and
decreases as we move away from the center to core
boundary. The thermal flux is increased near the
reflector because the neutron reflector scatters back
(or reflects) into the core many neutrons that would
otherwise escape. The neutrons reflected back into the
core are available for chain reaction (reflector
savings).
Keywords: Pebble-bed,
HTR-10, Flux distribution, power distribution,
thorium-based fuel.
doi.org/10.32006/eeep.2026.1.6874
RESEARCH
ON THE ALPHA OMEGA WATER® CRYSTAL INSTRUMENT: RESULTS AND ANALYSES WITH
FT–IR,
EIS,
NMR, AND DFT
Ignat Ignatov1, Yordan
G. Marinov2
1 Scientific Research
Center of Medical Biophysics (SRCMB), Sofia, Bulgaria
2 Georgi Nadjakov
Institute of Solid State Physics, Bulgarian Academy
of Sciences, Sofia, Bulgaria
Abstract: Alpha Omega Water® represents an
innovative approach within environmental engineering aimed
at improving the physicochemical properties of water as a
key natural resource. As a high-quality treated water
product, it is associated with processes that help
maintain cleaner, more environmentally friendly water
systems.
This study examines the properties of
Alpha Omega Water® using Fourier-transform infrared
spectroscopy (FT-IR), proton nuclear magnetic resonance
(NMR) spectroscopy, electrochemical impedance spectroscopy
(EIS), and quantum chemical calculations based on Density
Functional Theory (DFT). Treated water was compared with
untreated control samples to evaluate possible differences
in molecular organization and electrical behavior.
The results showed measurable
differences in spectroscopic characteristics, impedance
response, and modeled water-cluster parameters between
treated and control samples under the applied experimental
conditions.
These findings may be consistent with
differences in the local hydrogen-bond environment and the
associated physicochemical behavior of water.
These results indicate that Alpha Omega
Water® possesses properties relevant to processes that
improve the physicochemical characteristics of water,
thereby contributing to the maintenance of a cleaner, more
environmentally favorable water environment.
Keywords: Alpha Omega Water,
hydrogen bonds, water clusters, FT-IR, NMR, DFT, EIS
doi.org/10.32006/eeep.2026.1.7582
FEASIBILITY
OF INTEGRATING INTERNET OF THINGS (IoT) -ENABLED DOCKED
ELECTRIC SCOOTER SYSTEMS WITH PUBLIC TRANSPORT: A CASE
STUDY OF SOFIA, BULGARIA
Tsvetan Iliev
The Institute of Robotics, Bulgarian
Academy of Science (IR-BAS), Sofia, Bulgaria
Abstract: Urban transport systems face increasing
challenges related to congestion, emissions, and inefficient
first- and last-mile connectivity. This study proposes a
methodological framework for evaluating the integration of
Internet of Things (IoT)-enabled docked electric scooter
systems with urban public transport through a composite
index combining travel time, emissions, and cost. A case
study based on the transport network of Sofia, Bulgaria,
compares a conventional public transport scenario with an
integrated metro and shared e-scooter scenario. The results
demonstrate that the integrated approach can reduce travel
time by approximately 27% and transport-related emissions by
approximately 29%, while providing improved overall mobility
performance despite slightly higher direct user cost. The
proposed framework offers a simple and adaptable methodology
for evaluating integrated micromobility solutions in
sustainable urban transport systems.
Keywords:
micromobility, electric scooters, urban mobility, public
transport integration, IoT, sustainable transport
Teodora P. Popova1*,
Mila D. Kaleva2, Fabio Huether3,
Alexander I. Ignatov4, Ignat Ignatov4
1Faculty of Veterinary
Medicine, University of Forestry, 10 Kl. Ohridski Blvd.,
1756 Sofia, Bulgaria
2The
Stephan Angeloff Institute of Microbiology, Bulgarian
Academy of Sciences, 1113 Sofia, Bulgaria
3EVODROP AG, 8306
Brüttisellen, Switzerland
4Scientific Research
Center of Medical Biophysics (SRCMB), 1111 Sofia, Bulgaria
Email of
corresponding author: tpopova@ltu.bg
Abstract.
The
increasing prevalence of antimicrobial resistance has
intensified the search for safe and effective alternatives
to conventional antimicrobial agents, including natural
compounds. L-malic acid, a naturally occurring in fruits and
plant tissues, has attracted interest in this respect. The
present study evaluated the in vitro antimicrobial
activity of a formulation containing L-malic acid against
reference strains of Escherichia coli, Pseudomonas
aeruginosa, Staphylococcus aureus and Candida
albicans using a suspension assay. The tested
formulation comprised 60% (w/w) L-malic acid, 30% (w/w)
starch, and 10% (w/w) organosilica, obtained from product
IONSIEVE MALOX®. The formulation was evaluated at
nominal L-malic acid concentrations of 1, 2, and 3 mg·L-1
and exposure times of 1, 4, and 24 h using final inoculum
levels of 5×10² or 1×10² CFU/mL. Under the tested
conditions, the formulation reduced the number of
recoverable cells of all examined microorganisms in a
concentration-, time-, and inoculum-dependent manner. At 3
mg·L-1, no recoverable E. coli cells were
detected after 1 h at 5×10² CFU/mL, whereas C. albicans
reached undetectable levels after 4 h and P. aeruginosa
and S. aureus after 24 h. At 1×10² CFU/mL no
recoverable C. albicans cells were detected after 1
h, while in the other tested microorganisms - after 4 h. The
formulations at 1 and 2 mg·L-1 also reduced
recoverable counts of all tested strains, although with
weaker early effects. These findings indicate that, under in
vitro suspension-test conditions, L-malic acid
exhibited significant antimicrobial activity against the
tested bacterial and yeast strains and merit further
investigation under application-relevant conditions.
Keywords: L-malic acid, Escherichia coli,
Pseudomonas aeruginosa, Staphylococcus aureus, Candida
albicans, antimicrobial activity