EEEP 2/ 2022
H. Najdenski 3-4
How white biotechnology can contribute to bioeconomy?
J. M. François 5-17
Microbiological study of the anthropogenic pressure on the Yantra River in the region of Veliko Tarnovo
Z. Tsvetanova, D. Dimitrov, H. Naidenski 18-29
II. RENEWABLE AND ALTERNATIVE ENERGY SOURCES AND BIOFUELS
A theoretical overview of compressed air energy storage technologies and developments
L. Bakhtiari, D. Karamanev 30-44
III. BIOAUTOMATICS AND BIOINFORMATICS
Theoretical comparative studies of the biogaz yields from one-stage and two-stage anaerobic digestion processes
N. Pan, H. Wang, Y. Tian, E. Chorukova, I. Simeonov, N. Christov 45-55IV. SPACE TECHNOLOGY AND ENVIRONMENTAL MONITORING
Methodology for remote sensing monitoring of organic wheat crops
M. Chanev, L. Filchev, D. Valcheva 56-59
Bacterial degradation of cellulosic substrates under terrestrial and long term manned space mission conditions: a review (Part I)
L. Dimitrova, V. Hubenov, L. Kabaivanova, Y. Gocheva, P. Angelov, H. Najdenski 60-67
V. FOREST ECOLOGY AND BIOLOGY
Regimes for close-to-nature management of the high-mountain pastures on the territory of Belasitsa Nature Park
S. Savev 68-72
VI. RADIATION ECOLOGY
Assessment of the expected induced radioactivity in the bunker walls at the INRNE-BAS cyclotron facility
A. Demerdjiev, D. Tonev, N. Goutev, D. T. Dimitrov, G. Dimitrova, E. Geleva, S. Genchev 73-78
135 Avenue de Rangueil, F-31077 Toulouse, France;2Toulouse White Biotechnology center (TWB), UMS INRA –INSA,
135 avenue de Rangueil F31077-Toulouse, FranceAbstract. Biotechnology in its broadest sense is the application of science and technology to living organisms to produce goods, products and services. It is segmented into 10 branches, of which white biotechnology, also called industrial biotechnology, is the oldest, having its roots in the Neolithic period with the transformation of food into fermented products attributed to divine intervention at that time. White biotechnology really took off after Louis Pasteur demonstrated that fermentation is caused by living organisms. This discovery was followed in the early 20th century by the development of large-scale fermentation processes exploiting the intrinsic metabolic properties of microorganisms, such as solventogenesis in Clostridium or secondary metabolism for penicillin synthesis in Penicillium. With the advent of recombinant DNA in the 1970s, a new era of white biotechnology was born, with the ability to genetically manipulate microorganisms for the production of recombinant proteins and therapeutic agents, which notably boosted the biopharmaceutical sector. A third revolution in white biotechnology is nowadays occurring, driven by the strong societal demand to shift from a fossil fuel-based economy to one based on renewable carbon resources. The transition to so-called "bioeconomy" is expected to be slow and painful because it relies on the exploitation of "biological systems" that, unlike the chemical processes, are much more complex, inefficient, difficult to manage and still unpredictable. After a brief history of industrial biotechnology, I will present and address in this opinion paper some major challenges that await white biotechnology, using as an example our current work in the production of biosourced methionine, and I will discuss societal factors that could foster a bright future to white biotechnology in our modern society.
Leila Bakhtiari, Dimitre Karamanev
Department of Chemical and Biochemical Engineering,The University of Western Ontario, Canada
Abstract. In our modern world, technological developments on the one hand, and global warming and its consequences, on the other hand, cause us to feel the necessity for reliable, cost-effective, and clean energy. Using renewable energy sources paired with compressed air energy storage can be a good option that meets these expected criteria. Although a compressed air energy storage system (CAES) is clean and relatively cost-effective with long service life, the currently operating plants are still struggling with their low round trip efficiencies. This paper illustrates an up-to-date review of compressed air energy storage systems containing changes in the conventional process to improve performance and increase efficiency. Three main categories of compressed air energy storage technology, diabatic, adiabatic, and isothermal, are analyzed theoretically. In addition, three components of a compressed air energy storage system including compression system, reservoirs, and expansion system are discussed here in detail. The advantages, disadvantages, and the technological readiness of different types of CAES are discussed.Keywords: Compressed Air Energy Storage (CAES), Diabatic, Adiabatic, Isothermal.
Abstract. This paper deals with the theoretical comparison of biogas and energy yields of one-stage anaerobic digestion processes (OSAD) with biomethane production and two-stage anaerobic digestion processes (TSAD) producing biohydrogen and biomethane. The comparative study of the biogas yield from OSAD and TSAD systems is performed on the base on mathematical models obtained in our previous papers. The possible maximal yields of biohydrogen and biomethane are calculated by the static characteristics and extremum points of both systems. Simulation results suggest that in comparison to OSAD, the increase in biogas (biohydrogen and biomethgane) production of TSAD can reach to 75.18%. The energy produced from two-stage anaerobic digestion processes is 1.32 to 1.486 times greater than those from one-stage anaerobic digestion processes (depending of the inlet organics concentrations), which means TSAD is a better choice considering biogas and energy production.Keywords: anaerobic digestion, one-stage, two-stage, mathematical modeling, static characteristics, biogas yields, energy yield
Abstract: In the presented methodology for aerospace monitoring of autumn wheat crops, grown under the conditions of organic farming, the ways of applying ground and aerospace methods are discussed in detail. This includes field experiments, phenological observations, GIS and remote sensing methods and data (data from Sentinel-2 satellite and WingtraOne unmanned aerial vehicle with MicaSense RedEdge-MX multispectral camera and RGB camera) and statistical analyses. In order to achieve the aim and objectives of the study, an experiment was conducted on a organically certified production field sown with einkorn (Triticum monococum) in the period 2020-2021. The field is part of the holding of ET "Borislav Slavchev" in the village of Byala Reka, Parvomai Municipality, South-Central Bulgaria on the soil type of leached chernozem clays, with a size of 136 da.
Keywords: remote sensing, wheat crops, organic agriculture, methodology
Dimitrova1, Venelin Hubenov1,
Lyudmila Kabaivanova1, Yana Gocheva1,
Plamen Angelov2, Hristo Najdenski1
1 The Stephan Angeloff Institute of Microbiology at the Bulgarian Academy of Sciences
2 Space Research and Technology Institute at the Bulgarian Academy of Sciences
Abstract: The Earth and the lower atmosphere (troposphere and stratosphere) are constantly faced with numerous environmental challenges, one of which is the growing pollution due to the incineration of cellulose-containing waste with accumulating potential. In recent years scientists have focused on the complexity of ecological mechanisms in the biosphere of our planet - Earth, starting from laboratory, scaled and closed ecosystems. Onboard the spacecraft, textile products with antimicrobial properties are widely used which limits the spread of infections and ensures safety, comfort and resistance of the user. Another type of waste is the remains of sanitary and medical consumables, personal hygiene materials (e.g. wet and dry wipes, toilet paper, etc.), paper, inedible parts of greenhouse plants, etc., being usually subjected to microbial degradation. On Earth, the accumulation of these cellulose containing waste can cause serious environmental problems. Nowadays, many researchers are trying in experimental conditions on Earth to solve the problem of cellulose-containing waste by means of different approaches – burning, composting, burial, etc. The main risk and environmental problem is that the burial of waste in the soil and composting should contribute to the spread of microorganisms with pathogenic potential. Nevertheless, a promising approach is the microbial degradation of cellulose containing substrates realized by microbial consortia depending on the conditions of the surrounding environment. Therefore, the recent review aims to make a comparative analysis of the bacterial species involved in the degradation processes of cellulose-containing waste and to assess their potential for possible application in space conditions, including the International Space Station.
Keywords: cellulose-containing waste, biodegradation, microorganisms, life support systems, long-term manned space missions
ASSESSMENT OF THE EXPECTED INDUCED RADIOACTIVITY IN THE BUNKER WALLS AT THE INRNE-BAS CYCLOTRON FACILITY
Anguel Demerdjiev, Dimitar Tonev, Nikolay Goutev, Dobromir T. Dimitrov, Galina D. Dimitrova, Elena Geleva, Stefan G. Genchev
Institute for Nuclear Research and Nuclear Energy, Bulgarian Academy of SciencesAbstract. The Institute for Nuclear Research and Nuclear Energy at the Bulgarian Academy of Sciences is working on the construction of a cyclotron centre. Îne of the challenges in designing the radiation protection of a cyclotron centre is the preliminary assessment of the expected amounts of radioactive waste that will be generated during the lifetime of the facility. Our aim in this paper is to make an estimation of the expected induced radioactivity in the cyclotron bunker walls after the 20 year operational period of the facility. For this purpose, Monte Carlo simulations with the code FLUKA were performed. The distributions of the specific activity of the generated radionuclides in the concrete walls and of the residual dose rates inside the bunker are obtained. The specific activity of the most important long-lived radionuclides in the concrete walls of the bunker, namely 154Eu, 152Eu, 134Cs, 60Co, 54Mn is estimated. On the basis of these results an optimization of the shielding design is proposed in order to decrease the amount of radioactive waste which will be generated during the facility lifetime.