I. Simeonov 3
I. Human and biosphere
B. Mihaylov, M. Georgiev, A. Gadeleva 4
S. Kirilova, R. Nedkov, St. Modev 17
II. Microrganisms and environment
Z. Alexieva, M. Gerginova, H. Yemendzhiev 25
T. Avramova, M. Atanasova, A. Sotirova, D. Galabova 40
III. Technologies for depolution
Ya.Topalova, R. Dimkov, I. Ivanov, D. Kozuharov 49
IV. Renewable energy sources and biofuels
S. Mileva, K. Lahtchev 57
V. Bioinformatics and bioautomatics
A. Pandelova, N. Stoyanov, Tz. Romanska 65
N. Stoyanova 70
Prof. Nikolai Nikolov at the age of 80 76
NOISE MAPPING FOR SOFIA AGLOMERATION
Boris Mihaylov, Milcho Georgiev, Antonia Gadeleva
Abstract. The recent paper
presents in brief the realized from GIS–Sofia
Ltd and Spectri Ltd project for
Noise Maps for
Sofia Aglomeration (approved in 2010). Bellow
are cited the used methodology and instrumentation. In brief
are shown the overall
final results, and the main conclusions out of them.
Keywords: noise, noise map
ECOHYDROLOGICAL ANALYSIS ÎN THE RIVER VACHA BASIN, LIMITED TO TZANKOV KAMAK DAM, USING GIS ON THE BASE OF SATELLITE, GPS AND GROUND DATA
Silvia Kirilova, Roumen Nedkov, Stefan Modev
Abstract: In the paper the results from ecohydrological analysis conducted on the base of contemporary geoinformation technology are presented. The study and the analysis are made in GIS platform using satellite, GPS and ground data. Using GIS to solve the tasks of engineering hydrology gives the possibility/opportunity for a quick and easy access to data acquisition and analysis in space to be used. Thus, the influence of the subjective factor on the data and analysis reliability and precision is minimized.
Orohydrographical characteristics of the researched basin are the information base which together with run-off characteristics allow generalization and derivation of different regional empirical formulas. These empirical formulas are obtained under the analysis and estimations when defining the necessary parameters for the design of hydrotechnical facilities. The received empirical formulas are objective quantity ecological estimitation for further exploitation of hydrotechnical facilities.
Key words: Geographic Information System (GIS), GPS, Digital Elevation Model (DEM), river system, run-off
MICROBIAL DEGRADATION OF PHENOL AND PHENOLIC COMPOUNDS (REVIEW)
Zlatka Alexieva, Maria Gerginova, Husein Yemendzhiev
Abstract: Microorganisms are an important participant in the natural chemical cycles, but are also useful in the breakdown of chemical compounds produced by human activity. In the practice of cleaning the environment with microorganisms, most approved is their use in treatment plants for degradation of urban, agricultural or industrial waste. In the last 30-40 years in the environment fall within various highly toxic chemicals. Widespread application of physico-chemical methods of destruction are generally expensive and often lead to additional air pollution. With the development of biotechnology, it is clear that disposal of toxic pollutants by organic synthetic methods is more cost-effective and environmentally friendly. Phenol and its derivatives are known as highly toxic and widespread pollutants. The problem of effective removal from the environment is directly related to protecting the environment and human health.
Key words: phenol, biodegradation, bacteria, yeast, fungi, mathematical modeling
EFFECT OF RHAMNOLIPID BIOSURFACTANT ON CELL SURFACE PROPERTIES OF BACTERIAL STRAINS GROWN ON N-HEXADECANE
Tatyana Avramova, Maya Atanasova, Anna Sotirova, Danka Galabova
Abstract. Effect of biosurfactant rhamnolipid Ps-17, external to the studied ecosystem, on microbial cell growth, permeability and hydrophobicity of bacterial strains, members of a microbial consortium, isolated from lubricant-polluted water was studied. Bacterial strains were representatives of three genera, Enterococcus; Staphylococcus; Alcaligenes and were selected according to their ability to grow on n-hexadecane and to produce biosurfactant. It was shown that biosurfactant-rhamnolipid Ps-17 supported the growth of bacterial isolates on hexadecane in different degree. The results revealed that biosurfactant Ps-17 at used concentrations modified cell hydrophobicity and increased cell permeability of Gram (+) and Gram (-) isolates. Evaluation of the relationship between changes in cell surface permeability and hydrophobicity and the capability of the tested strains to utilize the hydrophobic carbon substrate n-hexadecane was made as well. The results obtained were discussed in attempt to elucidate the relationships between the members of the consortium and to evaluate the role of biosurfactants, self-produced or exogenous, in bioremediation of hydrocarbon-polluted sites.
Keywords: rhamnolipid; cell surface properties; n- hexadecane; Enterococcus; Staphylococcus; Alcaligenes
MODELING OF SHOCK LOADING WITH PCP-XENOBIOTIC DURING REAL WASTEWATER TREATMENT PROCESS
INVESTIGATIONS AND SELECTION OF SACCHAROMYCES CEREVISIAE STRAINS FOR BIO-ETHANOL MANUFACTURING
Silvia Mileva, Kantcho Lahtchev
Abstract. Eight strains of the yeast Saccharomyces cerevisiae were tested for their ability to produce ethanol under the stress conditions of elevated temperature of 370C and high amounts of glucose. Several criteria (dynamics of released CO2, amounts of ethanol generated, cell growth, amounts of residual sugars and productivity) were used for estimation of the strain performance. The results obtained revealed a group of four strains (∑1278b, RD-01, ¹4 è ¹27) that are resistant to the fermentation conditions employed and are perspective for further works aiming genetic improvement of their abilities.
Key words: bioethanol, alcohol fermentation, yeasts, Saccharomyces cerevisiae
TISSUE-BASED BIOSENSOR WITH INHIBITION FOR DETERMINATION OF ALDICARB
Antonia Pandelova, Nikolay Stoyanov, Tzvetomira Romanska
Abstract: In the present work biosensor system for determination of aldicarb is described. The biosensor transducer used as biological material plant tissue of potato Solanum tuberosum. The principle of measurements is based on inhibition of enzyme polyphenol oxidase by carbamatic acid type pesticide aldikarb. As basic substrate catechol is used. The experimental researches are carried out in three buffer solutions – distiller water, 0.025M phosphate buffer and 0.1M phosphate buffer. The best linear relationship between concentration of inhibitor and output signal is observed in 0.025M phosphate buffer. The experimental results of analytical characteristic of biosensor with different enzyme loading are given.
CLIMATICAL CONDITIONS AND VEGETATION SUCCESSIONS IN FORESTS OF “PARANGALITZA” RESERVE IN RILA Mts
Abstract. In the paper have been analyzed original data for the regeneration proceses in the “Parangalitza” reserve in Rila mountain and a climate data. The major part of the vegetation is primary vegetation and it is represented mainly by formation of Picea abies (L.) Karsten and formation of Abies alba Mill.; formation of Pinus silvestris L.and formation of Fagus sylvatica L. have lower frequency of occurrence. There are a different-aged regeneration structures of undergrowth established. The structure and species composition of undergrowth was determined in Picea abies ecosystems with some admixture of Abies alba and Fagus silvatica, and also in Pinus sylvestris ecosystems with some admixture of Picea abies. Based on the analysed results, a trend of vegetation succession was established.